Journal: iScience

Article Title: Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation

doi: 10.1016/j.isci.2022.105739

Figure Lengend Snippet: Basal expression of SLC7A5/SLC3A2 in the erythroid lineage (A) The panel represents the flow cytometry analysis showing the different splenic cell populations in basal conditions based on the surface expression of CD71 and Ter119 in control mice. SLC3A2 expression is also represented by a color code: yellow-orange representing the strongest expression and dark green-blue the weakest or absence of expression. (B) Histograms showing SLC3A2 expression in the different splenic CD71 HI /Ter119 NEG/LOW , CD71 HI /Ter119 HI , CD71 NEG /Ter119 HI and CD71 NEG/LOW /Ter119 NEG cell populations in control (in blue) and ErGFPcre Slc7a5 LoxP / LoxP mice (in green). The IgG control (in gray) is included. A representative experiment out of 23 is shown.

Article Snippet: Rabbit polyclonal Anti-LAT1 (SLC7A5) , Alomone Labs , Cat# ANT-105, RRID: AB_2756724.

Techniques: Expressing, Flow Cytometry, Analysis, Control, FACS

Journal: iScience

Article Title: Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation

doi: 10.1016/j.isci.2022.105739

Figure Lengend Snippet: SLC7A5/SLC3A2 expression in splenic erythroblasts during erythropoietic stress (A) Representative flow cytometry analysis showing the different splenic cell populations in control and PHZ-treated mice based on the surface expression of CD71 and Ter119: CD71 HI /Ter119 NEG/LOW , CD71 HI /Ter119 HI , and CD71 NEG/LOW /Ter119 NEG . (B) Relative Slc7a5 mRNA expression in the spleen of control (n = 8) and ErGFPcre Slc7a5 LoxP / LoxP (n = 3) mice in baseline conditions as well as control (n = 6) and ErGFPcre Slc7a5 LoxP / LoxP (n = 5) upon PHZ treatment. Data are shown as mean ± SEM. Statistical analysis was performed using a two-tailed Student’s t test with Welch’s correction to compare the groups as indicated in the figure (∗p < 0.05). (C) Histograms showing SLC3A2 expression in each of the populations indicated in untreated control mice (in blue) and control mice treated with PHZ (in red) are shown in left panels. In the right panels, the same histograms of the left panels are shown, but overlaying the histogram of SLC3A2 expression of ErGFPcre Slc7a5 LoxP/LoxP (in green) mice treated with PHZ. The IgG-APC control is presented in gray. For A and C a representative experiment out of 9 is shown. (D) Representative flow cytometry analysis of DRAQ5 expression versus forward scatter (FSC) of the splenic CD71 HI /Ter119 HI erythroid population of PHZ-treated control mice. Two CD71 HI /Ter119 HI populations differing in DRAQ5 signal can be distinguished, DRAQ5 HI and DRAQ5 LOW . The dashed line represents unlabeled cells. (E) Representative histograms of SLC3A2 expression in CD71 HI /Ter119 HI /DRAQ5 HI and CD71 HI /Ter119 HI /DRAQ5 LOW populations in control mice treated with PHZ (in red) and ErGFPcre Slc7a5 LoxP/LoxP (in green) mice treated with PHZ are shown. The negative signal of the IgG-PyC control is shown in gray. For D and E a representative experiment out of 7 is shown.

Article Snippet: Rabbit polyclonal Anti-LAT1 (SLC7A5) , Alomone Labs , Cat# ANT-105, RRID: AB_2756724.

Techniques: Expressing, Flow Cytometry, Analysis, Control, Two Tailed Test, FACS

Journal: iScience

Article Title: Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation

doi: 10.1016/j.isci.2022.105739

Figure Lengend Snippet: SLC7A5/SLC3A2 expression in circulating CD71 HI /Ter119 HI reticulocytes under anemic conditions and in response to rhEPO (A) Representative flow cytometry analysis showing circulating CD71 HI /Ter119 HI reticulocytes in PHZ-treated and control mice, as well as in mice subjected to phlebotomy. For PHZ a representative experiment out of 20 is shown. For phlebotomy, a representative experiment out of 8 is shown. (B) Representative histograms of SLC3A2 expression in circulating CD71 HI /Ter119 HI reticulocytes of untreated control mice (in blue), mice treated with PHZ or mice subjected to phlebotomy (in red), as well as ErGFPcre Slc7a5 LoxP/LoxP mice treated with PHZ or subjected to phlebotomy (in green). The IgG-APC control is represented in gray. Quantification of the mean fluorescence intensity (MFI) of SLC3A2 on circulating CD71 HI /Ter119 HI reticulocytes from control untreated mice (n = 33), control mice treated with PHZ (n = 20), ErGFPcre Slc7a5 LoxP/+ untreated mice (n = 11), ErGFPcre Slc7a5 LoxP/+ mice treated with PHZ (n = 10), ErGFPcre Slc7a5 LoxP/LoxP untreated mice (n = 16) and ErGFPcre Slc7a5 LoxP/LoxP mice treated with PHZ (n = 14). Quantification of the mean fluorescence intensity (MFI) of SLC3A2 on circulating CD71 HI /Ter119 HI reticulocytes from control mice (n = 8), control mice subjected to phlebotomy (n = 8), ErGFPcre Slc7a5 LoxP/+ untreated mice (n = 4), ErGFPcre Slc7a5 LoxP/+ mice subjected to phlebotomy (n = 4), ErGFPcre Slc7a5 LoxP / LoxP untreated mice (n = 3) and ErGFPcre Slc7a5 LoxP/LoxP mice subjected to phlebotomy (n = 3). (C) Western blot analyses of SLC7A5 relative to β-actin protein levels in circulating erythroid cells of control and ErGFPcre Slc7a5 LoxP/LoxP PHZ-treated mice. (D) L-phenylalanine uptake in isolated CD71 HI /Ter119 HI circulating reticulocytes from PHZ-treated control or ErGFPcre Slc7a5 LoxP/LoxP mice (n = 5). (E) Representative flow cytometry analysis of CD71 expression versus GYPA in healthy and anemic subjects (left panels). Histograms of SLC3A2 expression in circulating CD71 HI /GYPA HI and CD71 NEG /GYPA HI populations of control (blue) and anemic (red) subjects (right panels). A representative experiment out of 3 anemic subjects and 3 control subjects is shown. (F) Representative histograms of SLC3A2 expression in circulating CD71 HI /Ter119 HI reticulocytes of untreated control mice (blue) or mice treated with rhEPO (200U) (red). The IgG-APC control is represented in gray. Quantification of the mean fluorescence intensity (MFI) of SLC3A2 on circulating CD71 HI /Ter119 HI reticulocytes from untreated (n = 5) or rhEPO-treated control mice (n = 5), as well as from untreated (n = 3) or rhEPO-treated ErGFPcre Slc7a5 LoxP/LoxP mice (n = 5). Data are shown as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test when comparing more than two groups or two-tailed Student’s t test with Welch’s correction when comparing two groups (∗∗p < 0.01; ∗∗∗p < 0.005).

Article Snippet: Rabbit polyclonal Anti-LAT1 (SLC7A5) , Alomone Labs , Cat# ANT-105, RRID: AB_2756724.

Techniques: Expressing, Flow Cytometry, Analysis, Control, Fluorescence, Western Blot, Isolation, Two Tailed Test, FACS

Journal: iScience

Article Title: Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation

doi: 10.1016/j.isci.2022.105739

Figure Lengend Snippet: Mice lacking SLC7A5 in the erythroid lineage develop hypererythropoietinemia and smaller circulating RBCs (A) Serum EPO protein (pg/mL) in control mice (n = 18), ErGFPcre Slc7a5 LoxP/+ mice (n = 4), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 13) under baseline conditions; in control mice (n = 18), ErGFPcre Slc7a5 LoxP/+ mice (n = 9) and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 16) after PHZ treatment; and in phlebotomized control mice (n = 6), ErGFPcre Slc7a5 LoxP/+ mice (n = 4) and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 6). (B) Total number of circulating RBCs in control mice (n = 28), ErGFPcre Slc7a5 LoxP/+ mice (n = 13), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 18) under basal conditions or in control mice (n = 13), ErGFPcre Slc7a5 LoxP/+ mice (n = 9), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 13) after PHZ treatment. (C) Mean corpuscular volume (MCV, μm 3 ) of circulating RBCs in control mice (n = 28), ErGFPcre Slc7a5 LoxP/+ mice (n = 13), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 18) under basal conditions, and in control mice (n = 11), ErGFPcre Slc7a5 LoxP/+ mice (n = 7), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 12) after PHZ treatment. (D) Representative images of life imaging morphology cytometry (left) and 3D-cell rendering images (right) of RBCs of ErGFPcre Slc7a5 LoxP/LoxP and control mice. The RBC populations normally distributed their areas around a central average smaller for the ErGFPcre Slc7a5 LoxP / LoxP specimens than for control mice (central panels). Graph panels show the diameter (μm), area (μm 2 ), and elongation (%) of RBCs in control, and ErGFPcre Slc7a5 LoxP/LoxP mice. Data are shown as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test when comparing three groups or two-tailed Student’s t test with Welch’s correction when comparing two groups (∗p < 0.05; ∗∗∗p < 0.005).

Article Snippet: Rabbit polyclonal Anti-LAT1 (SLC7A5) , Alomone Labs , Cat# ANT-105, RRID: AB_2756724.

Techniques: Control, Imaging, Morphology, Cytometry, Analysis, Two Tailed Test

Journal: iScience

Article Title: Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation

doi: 10.1016/j.isci.2022.105739

Figure Lengend Snippet: Erythroid-SLC7A5 deficient mice have red blood cells with lower hemoglobin content (A) Mean corpuscular hemoglobin (MCH, pg) of circulating RBCs in control mice (n = 28), ErGFPcre Slc7a5 LoxP/+ mice (n = 13), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 18) under basal conditions. (B) Representative peripheral blood stainings with May-Grünwald-Giemsa of ErGFPcre Slc7a5 LoxP/LoxP (right) and control (left) mice. (C) Representative flow cytometry histograms comparing the phospho-rpS6 Ser235/6 signal in CD71 HI /Ter119 HI splenic erythroblasts of control and temsirolimus-treated mice (left panel). Representative flow cytometry histogram comparing the phospho-rpS6 Ser235/6 signal in CD71 HI /Ter119 HI splenic erythroblasts in control, and ErGFPcre Slc7a5 LoxP/LoxP mice (right panel). Quantification of phospho-rpS6 Ser235/6 signal from control (n = 6) and temsirolimus-treated mice (n = 5) (left panel). Quantification of phospho-rpS6 Ser235/6 signal from control (n = 9), ErGFPcre Slc7a5 LoxP/+ mice (n = 8), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 7) (right panel). The dashed line represents unlabeled permeabilized cells. Data are shown as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test when comparing three groups or two-tailed Student’s t test with Welch’s correction when comparing two groups (∗p < 0.05; ∗∗∗p < 0.005).

Article Snippet: Rabbit polyclonal Anti-LAT1 (SLC7A5) , Alomone Labs , Cat# ANT-105, RRID: AB_2756724.

Techniques: Control, Flow Cytometry, Analysis, Two Tailed Test, FACS

Journal: iScience

Article Title: Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation

doi: 10.1016/j.isci.2022.105739

Figure Lengend Snippet: Loss of SLC7A5 in the erythroid lineage increases the transition between circulating reticulocytes and mature erythrocytes (A) Flow cytometry histogram comparing the CD71 expressed by circulating Ter119 positive cells in a control, and ErGFPcre Slc7a5 LoxP/LoxP mice after PHZ treatment. Quantification of the proportion of CD71 HI /Ter119 HI , CD71 INT /Ter119 HI , and CD71 NEG /Ter119 HI in control (n = 35), ErGFPcre Slc7a5 LoxP/+ mice (n = 19), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 28) after PHZ treatment. The range of CD71 expression considered for the quantification of CD71 HI /Ter119 HI , CD71 INT /Ter119 HI , and CD71 NEG /Ter119 HI RBCs is indicated. (B) Flow cytometry histogram comparing the TMRM signal in circulating CD71 HI /Ter119 HI reticulocytes of control, and ErGFPcre Slc7a5 LoxP/LoxP mice after PHZ treatment. Quantification of TMRM signal from control (n = 7), ErGFPcre Slc7a5 LoxP/+ mice (n = 6), and ErGFPcre Slc7a5 LoxP/LoxP mice (n = 7) after PHZ treatment. The dashed line represents unlabeled cells. (C) Maximal mitochondrial oxygen consumption rate (OCR) in circulating cells of PHZ-treated mice (n = 14) and PHZ-treated ErGFPcre Slc7a5 LoxP/LoxP mice (n = 15). Data are shown as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test when comparing three groups or two-tailed Student’s t test with Welch’s correction when comparing two groups (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.005).

Article Snippet: Rabbit polyclonal Anti-LAT1 (SLC7A5) , Alomone Labs , Cat# ANT-105, RRID: AB_2756724.

Techniques: Flow Cytometry, Control, Expressing, Analysis, Two Tailed Test, FACS

Journal: iScience

Article Title: Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation

doi: 10.1016/j.isci.2022.105739

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal Anti-LAT1 (SLC7A5) , Alomone Labs , Cat# ANT-105, RRID: AB_2756724.

Techniques: Control, Amplification

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: SLC7A5 proteins are stained in brown; cell nuclei are counter stained purple. Original magnifications: 400×. ( A ) In benign mucosa, SLC7A5 expression is negative to very weak and localized to the cytoplasmic membrane or stromal cytoplasm. ( B-F ) In colorectal cancer, SLC7A5 is detected in tumor membrane at various levels, ranging from weakly positive tumor cells to strong expression in most cells. ( G, H ) SCL7A5 expression range distribution in 522 earl-stage cancers ( G ) and 81 late-stage cancers ( H ). Total weighted H-score of 40 is used as a cut-off (vertical red line). SLC7A5 expression in B, C are considered low and in D-F are considered high.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Staining, Expressing, Membrane

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: Correlation of SLC7A5 protein expression with clinicopathological features of colorectal cancer patients.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: High expression refers to cancer tissue with immunohistochemical staining H-score ≥40. ( A, B ) In early-stage cancers, SLC7A5 high expression is significantly correlated with longer survivals and better clinical outcome. Similar significant correlations hold in the MSS (microsatellite stability) and MSI (microsatellite instability) subtypes. ( C, D ) In late-stage cancers, SLC7A5 expression is not correlated with overall or disease-free survivals.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: Univariate and multivariate analyses of patient survival in early-stage colorectal cancer.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: The analysis was based on the mass spectrometry protein sequencing data from the CPTAC database. The Y-axis represents SLC7A5 protein level z-scores. SLC7A5 protein expression levels are significantly higher in BRAF-mutated tumors than wild-type tumors.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Mass Spectrometry, Sequencing, Expressing

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: ( A ) The analysis of the SLC7A5 protein and mRNA expressions in the 86-case CPTAC colorectal cohort revealed significant consistency between protein and gene transcription levels. Spearman’s rank correlation coefficient r = 0.5360, p<0.0001. ( B ) SLC7A5 mRNA expression showed the most significant positive correlation with SLC3A2 mRNA expression among all gene transcripts in the 592-case TCGA colorectal cancer cohort. Spearman’s rank correlation coefficient r = 0.7291, p<0.0001.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Protein expression of the amino acid transporter SLC7A5 in tumor tissue is prognostic in early-stage colorectal cancer

doi: 10.1371/journal.pone.0298362

Figure Lengend Snippet: Only those with Spearman’s rank correlation coefficient >0.4 were included in the network. Only interactions with high confidence (0.7/1.0) are shown in the figure. Amino acid transporters are colored (red). According to Gene Ontology Biological Process annotation, the SLC7A5-network is significantly enriched in ncRNA metabolic process (aqua), RNA metabolic process (blue), and cell cycle (green). Proteins/genes colored yellow are also commonly found in lymphocytes.

Article Snippet: Tissue sections were incubated with SLC7A5-specific polyclonal antibodies (HPA052673, 1:50, Atlas Antibodies, Sigma) for 30 min.

Techniques:

Journal: Cells

Article Title: Echinococcus granulosus -Induced Liver Damage Through Ferroptosis in Rat Model

doi: 10.3390/cells14050328

Figure Lengend Snippet: (A) Ultra-structure of hepatocytes near cysts at different stages of infection with PSCs (0, 1, 3, and 6 months); mitochondria are indicated by white arrows,PSC are indicated by black arrows( n = 3 rats/group, scale bars represent 2 μm and 1 μm). ( B ) Fe 2+ , ( C ) GSH, ( D ( a , b )) ROS, ( E ) MDA, ( F ) SOD, and ( G ) LDH of hepatocytes near cysts at different stages of infection with PSCs. ( H ( a , b )) Western blotting of protein expression levels of TFRC, GPX4, FTH1, NOX1, SLC3A2, and SLC7A11 in ferroptosis signaling pathway in rat liver tissue at different stages of infection with PSCs ( n = 3 rats/group)); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student t test).

Article Snippet: The antibodies used in this study were as follows: TFRC (Boster, 1: 500, Rabbit, BA0462-2, Wuhan, China); Caspase-3 (Boster, 1:500, Rabbit, BA3257, Wuhan, China); GSDMD (Proteintech Group, 1:800, Rabbit, 20770-1-AP, Wuhan, China); LC3I/II (Abcam, 1:1000, Rabbit, ab192890, Shanghai, China); Anti-GPX4 Antibody (Boster, 1:400, Rabbit, BM5231,Wuhan, China); Anti-FTH1 Antibody (Boster, China, 1:400, Rabbit, BM4487,Wuhan, China); Anti-NOX1 Antibody (Boster, China, 1:400, Rabbit, BA3720,Wuhan, China); Anti-CD98 Antibody (SLC3A2, Boster, China, 1:400, Rabbit, A01794-1,Wuhan,China); Anti-xCT Antibody (SLC7A11,Abcam,1:400, Rabbit, A01794-1, Shanghai, China); β-actin (Sino Biological, 1:1000, Mouse, 100166-MM10, Beijing, China).

Techniques: Infection, Western Blot, Expressing

Journal: Cells

Article Title: Echinococcus granulosus -Induced Liver Damage Through Ferroptosis in Rat Model

doi: 10.3390/cells14050328

Figure Lengend Snippet: ( A ) Fe 2+ in normal BRL cells, PSCs and BRL co-cultured cells, and PSCs +Ferrostatin-1 and BRL co-cultured cells. ( B ) GSH, ( C ) GSSH, ( D ) GSH/GSSH, ( E ) SOD, ( F ) LDH, and ( G ) MDA concentration detection ( n = 6 rats/group). ( H ( a , b )) Western-blotting detection of expression levels of cell ferroptosis signaling pathway proteins in each group, such as TFRC, GPX4, FTH1, NOX1, SLC3A2, andSLC7A11 ( n = 3 rats/group); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student t test).

Article Snippet: The antibodies used in this study were as follows: TFRC (Boster, 1: 500, Rabbit, BA0462-2, Wuhan, China); Caspase-3 (Boster, 1:500, Rabbit, BA3257, Wuhan, China); GSDMD (Proteintech Group, 1:800, Rabbit, 20770-1-AP, Wuhan, China); LC3I/II (Abcam, 1:1000, Rabbit, ab192890, Shanghai, China); Anti-GPX4 Antibody (Boster, 1:400, Rabbit, BM5231,Wuhan, China); Anti-FTH1 Antibody (Boster, China, 1:400, Rabbit, BM4487,Wuhan, China); Anti-NOX1 Antibody (Boster, China, 1:400, Rabbit, BA3720,Wuhan, China); Anti-CD98 Antibody (SLC3A2, Boster, China, 1:400, Rabbit, A01794-1,Wuhan,China); Anti-xCT Antibody (SLC7A11,Abcam,1:400, Rabbit, A01794-1, Shanghai, China); β-actin (Sino Biological, 1:1000, Mouse, 100166-MM10, Beijing, China).

Techniques: Cell Culture, Concentration Assay, Western Blot, Expressing

Journal: PLOS One

Article Title: Identification of differentially expressed genes associated with ferroptosis in ulcerative colitis

doi: 10.1371/journal.pone.0327990

Figure Lengend Snippet: (A) Chord diagram of the correlations of the five hub genes. (B) The expression of SLC7A5 was positively correlated with PSAT1. (C) SLC7A5 was positively correlated with SLC7A11. (D) SLC7A5 expression was negatively correlated with ACSF2 expression. (E) There was a moderate negative correlation between SLC7A11 and ACSF2 expression. (F) There was a moderate negative correlation between PSAT1 and ACSF2 expression. (G) There was a moderate negative correlation between ACSL4 and ACSF2 expression.

Article Snippet: Subsequently, the sections were blocked with goat serum for 30 minutes at room temperature.Following the blocking step, the sections were incubated overnight at 4°C with primary antibodies targeting SLC7A5 (Sanying, Wuhan, China).

Techniques: Expressing

Journal: PLOS One

Article Title: Identification of differentially expressed genes associated with ferroptosis in ulcerative colitis

doi: 10.1371/journal.pone.0327990

Figure Lengend Snippet: (A) GSE87466 , volcano plot shows the expression distribution of DEGs in the dataset. Based on adj. P < 0.05 and | logFC | 1 or higher standard of truncation, dot said its genes, and the red dot raised genes, SLC7A11, PSAT1, SLC7A5, ACSL4 and ACSF2 expression to display. (B-F) ROC curve analysis of central genes ACSF2, SLC7A5, ACSL4, SLC7A11 and PSAT1.

Article Snippet: Subsequently, the sections were blocked with goat serum for 30 minutes at room temperature.Following the blocking step, the sections were incubated overnight at 4°C with primary antibodies targeting SLC7A5 (Sanying, Wuhan, China).

Techniques: Expressing

Journal: PLOS One

Article Title: Identification of differentially expressed genes associated with ferroptosis in ulcerative colitis

doi: 10.1371/journal.pone.0327990

Figure Lengend Snippet: (A) Histogram: Comparison of RT-qPCR monitoring of HUB genes expression in the intestines of normal and UC mice; (B) Western blot analysis of SLC7A5 expression levels in the intestines of normal and UC mice; (C) Box plot: Depiction of SLC7A5 protein expression levels in the normal and DSS groups; (D) Representative image of immunohistochemical staining depicting SLC7A5 level in intestine tissue of normal mice, scale bar,20µm; (E) Representative image of immunohistochemical staining depicting SLC7A5 level in colon tissue of UC mice, scale bar,20µm; (F) Histogram: Comparison of immunohistochemical staining for SLC7A5 expression in the intestines of normal and UC mice. ***P < 0.001.

Article Snippet: Subsequently, the sections were blocked with goat serum for 30 minutes at room temperature.Following the blocking step, the sections were incubated overnight at 4°C with primary antibodies targeting SLC7A5 (Sanying, Wuhan, China).

Techniques: Comparison, Quantitative RT-PCR, Expressing, Western Blot, Immunohistochemical staining, Staining

Journal: PLOS One

Article Title: Identification of differentially expressed genes associated with ferroptosis in ulcerative colitis

doi: 10.1371/journal.pone.0327990

Figure Lengend Snippet: (A) Normal group intestinal histology under colonoscopy; (B) UC patient intestinal histology under colonoscopy; (C) Histological images of normal group intestinal mucosa stained with H&E, scale bar,100µm; (D) Histological images of UC patient intestinal mucosa stained with H&E, scale bar,100µm; (E) Representative image of immunohistochemical staining depicting SLC7A5 level in colon tissue of normal group, scale bar,20µm; (F) Representative image of immunohistochemical staining depicting SLC7A5 level in colon tissue of UC patient, scale bar,20µm; (G) Histogram: Comparison of immunohistochemical staining for SLC7A5 expression in the intestines of normal and UC patients. ***P < 0.001.

Article Snippet: Subsequently, the sections were blocked with goat serum for 30 minutes at room temperature.Following the blocking step, the sections were incubated overnight at 4°C with primary antibodies targeting SLC7A5 (Sanying, Wuhan, China).

Techniques: Staining, Immunohistochemical staining, Comparison, Expressing